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ProteomicsWithanolide analogues disrupt a leukemic dependency on cholesterol transport by inhibiting the oxysterol-binding protein OSBP (TPP)
Metabolic alterations in cancers precipitate in associated dependencies that can be therapeutically exploited. To meet this goal, natural product inspired small molecules can provide a resource of underexplored chemotypes. We identify W7, a synthetic withanolide analog with pronounced anti-leukemic ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-27 | PXD040692 | Pride
Thermal proteome profiling Expression proteomics Withanolides Natural product-inspired compound libraries 
ProteomicsTAK285 induced degradation – selectivity profiling in NALM-6 cells
The hit compound TAK285 was subjected to full proteome profiling by TMTpro 6-plex to assess its degradation selectivity in NALM-6 cells. TAK285 induced the degradation of the kinase BLK. The compound was further mechanistically dissected as reported with orthogonal assays such as FACS-based CRISPR/C...
ORGANISM(S): Homo sapiens (Human) 
2025-09-04 | PXD059599 | Pride
Kinase inhibitors Tmt 
ProteomicsProbing kinase proximity interactome modulation upon drug treatment by miniTurboID proximity labelling
In a systematic effort to profile kinase inhibitors, which also act as degraders, we identified multiple hit compounds. These were subjected to detailed mechanism of action elucidation including analysis of the perturbed interactome and its effect on degradation. For our first example, LYN, Src inhi...
ORGANISM(S): Homo sapiens (Human) 
2025-09-04 | PXD062184 | Pride
Kinase inhibitors Tmt 
ProteomicsWithanolide analogues disrupt a leukemic dependency on cholesterol transport by inhibiting the oxysterol-binding protein OSBP (Expression proteomics)
Metabolic alterations in cancers precipitate in associated dependencies that can be therapeutically exploited. To meet this goal, natural product inspired small molecules can provide a resource of underexplored chemotypes. We identify W7, a synthetic withanolide analog with pronounced anti-leukemic ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-27 | PXD040694 | Pride
Thermal proteome profiling Expression proteomics Withanolides Natural product-inspired compound libraries 
ProteomicsSelectivity profiling of inhibitor treated human cells via full proteome profiling
Three selective hit compounds from an arrayed kinome abundance screen (98 kinases vs 1570 kinase inhibitors) were subjected to full proteome profiling by TMTpro 18-plex to assess their degradation selectivity in K562 cells. Src inhibitor 3 showed the most significant downregulation of the target kin...
ORGANISM(S): Homo sapiens (Human) 
2025-06-09 | PXD053130 | Pride
K562 Kinase inhibitors Monovalent degrader Tmt 
TranscriptomicsGenome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment
In this study, a newly developed 8x15K Fusarium graminearum Agilent microarray was applied to analyze transcriptional responses to azole fungicide treatment. Comparative statistical analysis of expression profiles from treated versus untreated fungal liquid cultures uncovered 1058 genes that were si...
ORGANISM(S): Gibberella zeae 
2011-01-26 | E-GEOD-25114 | biostudies-arrayexpress
ProteomicsMetaproteome of the waste water treatment plant Gerwisch/Germany
In this study the impact of protein fractionation techniques prior to LC/MS analysis was investigated on activated sludge samples derived at winter and summer condition from a full-scale wastewater treatment plant (WWTP). For reduction of the sample complexity, different fractionation techniques inc...
ORGANISM(S): Bacteria 
2015-07-28 | PXD001547 | Pride
Metaproteomics Activated sludge Metaproteomeanalyzer Wastewater treatment plant Offgel isoelectric focusing 
TranscriptomicsTranscriptome data of interacting Salmonella Typhimurium and human host cells
The poly(A)+ and poly(A)− fractions of interacting and non-interacting cells were used for distinct library preparation of interacting and non-interacting prokaryotic pathogen and eukaryotic host cells by deepSuperSAGE. Sequencing was performed with the Illumina HiSeq 2000 platform, and one point of...
ORGANISM(S): Salmonella enterica 
2015-05-12 | E-GEOD-61730 | biostudies-arrayexpress
ProteomicsSelectivity profiling of SLC16A3/MCT4 inhibitor slCeMM1 using photoaffinity probe
To assess the selectivity of SLC16A3/MCT4 inhibitor slCeMM1, we derived diazirine-alkyne photoaffinity probe (slCeMM1-PAP). We next used HAP1 and MDAMB231 cell lines for MS experiment, comparing the enrichment of proteins by slCeMM1-PAP and conditions where slCeMM1-PAP was competed with slCeMM1 or i...
ORGANISM(S): Homo sapiens (Human) 
2023-10-16 | PXD040089 | Pride
Photoaffinity probe  ap-ms  tmt  chemoproteomics 
ProteomicsThe Proteome Landscape of the Kingdoms of Life
Proteins preform the vast majority of functions in all biological domains but their large-scale investigation has lagged behind for technological reasons. Since the first essentially complete eukaryotic proteome was reported1, advances in mass spectrometry (MS)-based proteomics2 have enabled increas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-07-13 | MSV000085728 | MassIVE
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