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A new workflow for metaproteomics is presented in this study and is shown to enable the analysis of a broad range of different samples in only 24 h. The standardized sample preparation includes the combined cell lysis and phenol extraction in a ball mill followed by FASP digestion. The bioinformatic...
ORGANISM(S): Enterobacteriaceae sp. Methanosarcinales 
2019-09-04 | PXD010550 | Pride
Within the scope of a technical note we tested our MetaProteomeAnalyzer Portable software workflow on experimental data sets from samples with known composition. For generating a benchmarking data set, the bacterial strains (5BCT) Bacillus subtilis, Escherichia coli, Pseudomonas fluorescens, Microco...
ORGANISM(S): Pseudomonas fluorescens Escherichia coli Micrococcus luteus (Micrococcus lysodeikticus) Desulfovibrio vulgaris str. Hildenborough Bacillus subtilis 
2012-12-13 | PXD007681 | Pride
Objective Crohn’s Disease (CD) and Ulcerative Colitis (UC) are chronic inflammatory diseases of the gastrointestinal tract. Reliable diagnosis of these diseases requires a comprehensive examination of the patient, which include invasive endoscopy. This study assesses whether non-invasive LC-MS/MS ba...
ORGANISM(S): Homo sapiens (Human) 
2019-03-11 | PXD010371 | Pride
Biogas plants (BGPs) produce methane and carbon dioxide through the anaerobic digestion of agricultural waste. Identification of strategies for more stable biogas plant operation and increased biogas yields require better knowledge about the individual degradation steps and the interactions within t...
ORGANISM(S): environmental samples <Methanosarcinales> 
2019-05-03 | PXD009349 | Pride
In this study the impact of protein fractionation techniques prior to LC/MS analysis was investigated on activated sludge samples derived at winter and summer condition from a full-scale wastewater treatment plant (WWTP). For reduction of the sample complexity, different fractionation techniques inc...
ORGANISM(S): Bacteria 
2015-07-28 | PXD001547 | Pride
Phages are viruses that infect prokaryotes and can shape microbial communitiesby lysis, thus offering applications in various fields. However, challengesexist in sampling, isolation and accurate prediction of the host specificity ofphages as well as in the identification of newly replicated virions ...
ORGANISM(S): Escherichia phage Lambda 
2024-08-29 | PXD044316 | Pride
Metatranscriptomic and metaproteomic analysis of C.quadricolor symbiotic bacteria for discovery of new potential biosynthetic clusters
ORGANISM(S): metagenome Chromodoris quadricolor 
2022-12-07 | PXD037957 | Pride
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