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ProteomicsMembrane proteome of renal BBMVs from the mouse model
In order to demonstrate the efficacy of the membrane enrichment method by Urea Wash in the animal model. Kidneys were dissected from 8 weeks old male mice of inbred strain C57BL/6J and flash-frozen in liquid nitrogen followed by storage at -80 °C. Brush border membrane vesicles (BBMV) were prepared...
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD027474 | JPOST Repository
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsMembrane proteome of HEK293T cells
We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enr...
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027473 | JPOST Repository
ProteomicsProteomics analysis of iPS spheres
Proteomics analysis of membrane proteins extracted from iPS spheres. Membrane fractions were isolated, treated with 8 M urea, and trypsinized. The peptides were fractionated (4 fractions) and analyzed by LC-MS/MS (Q-Exactive and UHPLC).
ORGANISM(S): Homo Sapiens (human) 
2025-08-05 | PXD065256 | JPOST Repository
ProteomicsInteractome of DYRK2 by using BioID proximity labeling
In this study, DYRK2 was found to be an important regulator of Hedgehog signaling. To identify DYRK2 interacting proteins, we performed interactome using BioID proximity labeling. NIH3T3 cells containing Dox-inducible DYRK2-BioID2-HA construct were cultured in the media with or without Doxycycline ...
ORGANISM(S): Mus Musculus (mouse) 
2024-07-01 | PXD043445 | JPOST Repository
ProteomicsProteome analysis of brainstem organoids from human pluripotent stem cells
Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development. We applied proteomic analysis to human brainstem organoids which were developed by a novel method.
ORGANISM(S): Homo Sapiens (human) 
2020-05-01 | PXD016807 | JPOST Repository
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