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ProteomicsSILAC-labeled proteins in ribosome samples
we developed a novel method combining axon-pSILAC17 with axonal ribosome purification. In this method, we first labeled newly synthesized proteins with heavy amino acids (AAs) in somaless axons cultured in a Boyden chamber. Approximately 2000 eyes were cultured for each experiment to obtain sufficie...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-12-09 | PXD011032 | Pride
Xenopus laevis Axon Ribosome Silac 
ProteomicsRegulated local synthesis of ribosomal proteins maintains ribosome function in axons
Ribosome assembly occurs mainly in the nucleolus yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-12-09 | PXD015574 | Pride
Ribosomes Sucrose density gradient fractionation Axon 
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