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ProteomicsDiscovery, target identification and lead optimisation of a quinazoline series with potent anti-Chagasic activity
We next used an approach to determine the direct binding of quinazolines to their target(s). A protein can be stabilised or destabilised through the binding of a ligand, altering that proteins thermostability. Such alterations in thermostability can cause a ligated protein to denature at a different...
ORGANISM(S): Trypanosoma cruzi Dm28c 
2025-04-22 | PXD050235 | Pride
Drug target Trypanosoma cruzi Thermal proteome profiling 
ProteomicsUbiquitination in treated T. cruzi parasites
T. cruzi epimastigotes in the logarithmic growth phase (3×106 cells mL-1) were incubated for 12h with bortezomib (1.8 µM), GNF6702 (2.9 µM) or compound 1 (24 µM), equivalent to 8× the EC50 values of each compound. Controls were incubated in the presence of diluent (DMSO). Cells were harvested by cen...
ORGANISM(S): Trypanosoma cruzi Dm28c 
2022-02-17 | PXD027524 | Pride
Ubiquitination Trypanosoma cruzi Proteasome 
ProteomicsMultiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial
Samples were reduced by the addition of TCEP (25 mM final concentration) and incubated at 37 ºC for 10 min. Alkylation was carried out by the addition of iodoacetamide (25 mM final concentration) and incubated at RT for 1 h in the dark. Samples were then digested by the addition of 1:50 LysC (Wako, ...
ORGANISM(S): Leishmania donovani BPK282A1 
2021-07-18 | PXD023780 | Pride
Thermal proteome profiling Leishmania Oxidosqualene synthase 
ProteomicsTarget deconvolution in Plasmodium falciparum using pulldowns
Parasite lysates were centrifuged (20,000 g, 4°C, 15 min). Beads (blank and with linker attached) were washed 3× with water then twice with lysis buffer. The lysate (~2 mg total protein) was first incubated with 2 mg blank beads for 30 min at 4°C with rotating agitation. The lysate was divided into ...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-10-15 | PXD033740 | Pride
Pulldown Plasmodium falciparum Drug target Chemical proteomics 
ProteomicsDrug target deconvolution in Plasmodium falciparum using thermal proteomics profiling
Early identification of a compound’s mode of action can greatly benefit the discovery process. Thermal proteomics profiling (TPP) is a powerful, unbiased tool that can be used to identify potential ligands of protein targets. TPP takes advantage of the fact that a ligand binding to its target prot...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-10-13 | PXD025182 | Pride
Lysine trna synthetase Thermal proteome profiling Plasmodium falciparum 
ProteomicsSterol 14-alpha demethylase (CYP51) activity in Leishmania donovani is likely dependent upon cytochrome P450 reductase 1
Liposomal amphotericin B is an important frontline drug for the treatment of visceral leishmaniasis, a neglected disease of poverty. The mechanism of action of amphotericin B (AmB) is thought to involve interaction with ergosterol and other ergostane sterols, resulting in disruption of the integrity...
ORGANISM(S): Leishmania donovani BPK282A1 
2024-06-21 | PXD052472 | Pride
Visceral leishmaniasis Mechanism of action P450 reductase and sterol 14-alpha demethylase (cyp51) Drug resistance Leishmania Sterol metabolism 
Genomicsgenotyped_bacterial_meningitis
This study includes 1146 samples of host genotyping data (genotyped) from Illumina Omni arrays. Samples were collected from adults (>16 yrs) patients with CSF confirmed bacterial meningitis in the Netherlands between 2006 and 2015. Metadata includes patient outcome, species of bacteria, and for 4...
EGAD00010002328 | EGA
Genomicsimputed_bacterial_meningitis
This study includes 1146 samples of host genotyping data (imputed) from Illumina Omni arrays, using https://imputation.sanger.ac.uk/ with the Haplotype Reference Consortium v1.1. Samples were collected from adults (>16 yrs) patients with CSF confirmed bacterial meningitis in the Netherlands betwe...
EGAD00010002327 | EGA
GenomicsHost genotyping data from Dutch adult bacterial meningitis patients and linked bacterial genome sequences
Streptococcus pneumoniae is a common nasopharyngeal colonizer, but can also cause life-threatening invasive diseases such as empyema, bacteremia and meningitis. Genetic variation of host and pathogen is known to play a role in invasive pneumococcal disease, though to what extent is unknown. This st...
EGAS00001005993 | EGA
GenomicsMeningene researchers data access committee
Data Access Committee EGAC00001002513
EGAC00001002513 | EGA
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