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MetabolomicsNew insights into the responses of soil microorganisms to polycyclic aromatic hydrocarbon stress by combining enzyme activity and sequencing analysis with metabolomics
Polycyclic aromatic hydrocarbons (PAHs), some of the most widespread organic contaminants, are highly toxic to soil microorganisms. Whether long-term polluted soils can still respond to the fresh input of pollutants is unknown. In this study, the soil enzyme activity, soil microbial community struct...
2021-02-17 | MTBLS1196 | MetaboLights
TranscriptomicsExpression analysis of Tim-3 knockdown RAW264.7 cell
Investigation of whole genome gene expression level changes in a Tim-3 knockdown RAW264.7 cell, compared to control siRNA transfected RAW264.7 cell. The Tim-3 knockdown of this cell render it hyper-reactive to TLR stimulation. Tim-3 knockdown analyzed in this study are further described in Li Y, Fen...
ORGANISM(S): Mus musculus 
2012-12-05 | E-GEOD-42729 | biostudies-arrayexpress
ProteomicsMass spec results of Tmc7-GST pull-down
For the GST pull-down of DRG ganglion, the GST- or Tmc7-GST-associated beads were further incubated with cell lysates derived from DRG ganglion. The pulled-down proteins were then separated by SDS-PAGE gel electrophoresis and subsequently stained with silver staining Kit. Finally, the target molecul...
ORGANISM(S): Mus Musculus 
2024-03-08 | PXD050466 |
ProteomicsLockd promotes myoblast proliferation and skeletal muscle regeneration through binding with DHX36 to facilitate unwinding of 5’UTR rG4 and translation of Anp32e mRNA
Adult muscle stem cells, also known as satellite cells (SCs) play pivotal roles in injury induced muscle regeneration and our knowledge of long non-coding RNA (lncRNA) functions in SCs remains limited. Here we identify a lncRNA, Lockd which is induced in activated SCs upon acute muscle injury. We de...
ORGANISM(S): Mus Musculus 
2022-05-24 | PXD033658 |
ProteomicsArginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells
m6A is the most abundant modification of mRNA in mammals and plays an important role in human development and disease. METTL14 is a key component of m6A methyltransferase complex. The project showed that its post-translational arginine methylation can regulate the generation of m6A and the endoderm ...
ORGANISM(S): Homo Sapiens 
2021-05-17 | PXD018458 |
ProteomicsRaw data of 218 FFPE tissue proteomes containing 144 tissue samples from seven organs of fatal VID-19 cases and 74 control specimens from 56 non-COVID-19 individuals
144 tissue samples from postmortem lung, liver, kidney, heart, spleen, testis and thyroid collected from 19 COVID-19 cases and 74 control specimens from 56 non-COVID-19 individuals were analyzed by PCT and TMT 16-plex based proteomics.
ORGANISM(S): Homo Sapiens 
2020-12-11 | PXD022775 |
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