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ProteomicsxIP-MS: topological analysis of chromatin-associated protein complexes using single affinity purification
In recent years, cross-linking mass spectrometry (XL-MS) has proven to be a robust and effective method of interrogating macromolecular protein complex topologies at peptide resolution. Traditionally, XL-MS workflows have utilized homogenous complexes obtained through time-limiting reconstitution, t...
ORGANISM(S): Homo sapiens (Human) 
2015-11-17 | PXD002987 | Pride
Affinity purification Chemical cross-linking mass-spectrometry Chromatin 
UnknownCross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification.
Not available
S-EPMC4813705 | biostudies-literature
ProteomicsComprehensive cross-linking mass spectrometry reveals molecular insights into the between peripheral and core NuRD complex subunits
Affinity purifications of different subunits of the NuRD complex, followed by cross-linking mass spectrometry (xIP-MS) to identify interaction domains. Six subunits and an interactor of NuRD were cross-inked with either BS3 or ADH/DMTMM and digested with either trypsin or chymotrypsin, to generate a...
ORGANISM(S): Homo sapiens (Human) 
2020-12-07 | PXD017244 | Pride
Mbd Chd Xip-ms Cross-linking Nurd 
UnknownCross-linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex.
Not available
S-EPMC8246863 | biostudies-literature
ProteomicsProteomic analysis revealed the potential role of MAGE-D2 in therapeutic targeting of triple-negative breast cancer
Triple negative breast cancer (TNBC) is one of the most aggressive molecular subtypes in breast cancer. Due to the lack of effective therapeutic targets, the clinical outcomes for TNBC patients remain poor. Here, we performed an integrated proteomic analysis including bioinformatic analysis of prote...
ORGANISM(S): Homo Sapiens 
2023-03-07 | PXD040645 |
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-10 | PXD011323 | Pride
Interaction proteomics Low input Ap-ms 
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs usi...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012800 | Pride
Interaction proteomics Low input Ap-ms 
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