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ProteomicsAffinity purification of biotinylated AtACINUS and Mass Spectrometric analysis to identify PTMs on AtACINUS
AtACINUS protein is involved in regulation of alternative transcription and splicing (AS). Previously we have identified O-GlcNAcylation and phosphorylation sites on AtACINUS using LWAC and IMAC enrichment (Xu et al., 2017). To identify more modification sites, we have created AtACINUS-YFP-TurboID/a...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-12-02 | PXD020749 | Pride
Seedling Acinus Plant Co-immunoprecipitation Arabidopsis thaliana atacinus O-glcnacylation Ip-ms Phosphorylation O-fucosylation Lc-ms/ms 
ProteomicsQuantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F2 R1 on QE-HF)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discove...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-22 | PXD044831 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsQuantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F1 on Eclipse (EThcD)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discove...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-22 | PXD044832 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsGlobal-scale enrichment and identification of O-GlcNAc modified peptides in Arabidopsis seedling (S1 Part 1: HCD and EThcD part from Eclipse)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. However, our understanding of this PTM in plants lags behind animals. To bridge this knowledge gap, we performed a global scale enrichment of O...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD044701 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsGlobal-scale enrichment and identification of O-GlcNAc modified peptides in Arabidopsis seedling (Part 1: HCD part from Eclipse)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. However, our understanding of this PTM in plants lags behind animals. To bridge this knowledge gap, we performed a global scale enrichment of O...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-29 | PXD044770 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsQuantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F1 on QE-HF) and Eclipse (HCD)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discove...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD044668 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsGlobal-scale enrichment and identification of O-GlcNAc modified peptides in Arabidopsis seedling (S1 Part 1: HCD from QE)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. However, our understanding of this PTM in plants lags behind animals. To bridge this knowledge gap, we performed a global scale enrichment of O...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD044675 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsMapping Architecture of Protein complexes in Arabidopsis using XL-MS (Part I Cell lysate)
Capturing molecular machines in action is key to understanding protein complex architecture, cellular regulation, and gene function. Here, we present a cross-linking mass spectrometry (XL-MS) platform using the PhoX cross-linker to map proteome-wide interactions in Arabidopsis thaliana. By analyzing...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-29 | PXD066234 | Pride
ProteomicsMapping Architecture of Protein complexes in Arabidopsis using XL-MS (Part III Nucleus_R1&R2)
Capturing molecular machines in action is key to understanding protein complex architecture, cellular regulation, and gene function. Here, we present a cross-linking mass spectrometry (XL-MS) platform using the PhoX cross-linker to map proteome-wide interactions in Arabidopsis thaliana. By analyzing...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-29 | PXD073734 | Pride
ProteomicsMapping Architecture of Protein complexes in Arabidopsis using XL-MS (Part II Chloroplast)
Capturing molecular machines in action is key to understanding protein complex architecture, cellular regulation, and gene function. Here, we present a cross-linking mass spectrometry (XL-MS) platform using the PhoX cross-linker to map proteome-wide interactions in Arabidopsis thaliana. By analyzing...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-29 | PXD066291 | Pride
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