THP-1 macrophages were infected with four strains of Mycobacterium tuberculosis to study the temporal dynamics of newly synthesized proteins in the secretome. Temporal snapshots of secretome reflect the macrophage response to pathogenicity which in combination with intracellular events, completes th...
we performed proteome analysis of Th9 cells to understand the involvement of proteins that might be crucial for the anti-tumor functions of Th9 cells. Here we show for the first time a comprehensive proteomic analysis of murine Th0 and Th9 cells, which identified 1451 total proteins among which 1367...
Since we have found that the RhoC gene causes extensive changes to gene expression, a methylation array was carried out to determine if the observed transcriptional changes are due to methylation changes in the genome.
The aim of the experiment was to identify regions of DNA bound by RhoC and WDR5. SiHa cells overexpressing RhoC were crosslinked, digested with MNase and sonicated. The fragmented DNA that bound to RhoC and WDR5 was pulled down using RhoC and WDR5 antibodies and sequenced using the Illumina HiSeq pl...
Since RhoC is known to be involved in various tumour phenotypes, a transcriptome analysis was carried out with RhoC over-expressing cells to identify the genes altered by RhoC over-expression.
