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This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, mic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-29 | MSV000086385 | MassIVE
We propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMinerTM is an equalizing technique by reducing high-abundance proteins and enriching low-abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMinerTM enrichment and peptide fractionat...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-25 | MSV000081649 | MassIVE
Salt stress poses a major challenge to plant growth and restricts agricultural development. In order to further study the complex mechanism of soybean response to salt stress, this study explored the pathways and regulatory networks of soybean salt tolerance from transcriptomics, proteomics and meta...
ORGANISM(S): Glycine max 
2025-05-07 | PXD052320 | Pride
Our goal is the identification of more and more the missing proteins from cancer cell lines. This project focuses on the membrance proteins separated by the ultracentrifuge and the low abundant peptides enriched by the Proteominer Kit and the hydrophobic peptides extracted by the organic solvent Ace...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-26 | MSV000086369 | MassIVE
Urine is an ideal material to study and examine the bladder cancer (BCa) biomarkers, whereas exploration to the corresponding protein candidates is confronting technique challenges. Herein, we proposed a comprehensive strategy of searching the urine proteins related to BCa. The strategy consists of ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080696 | MassIVE
Our goal is the identification of more and more missing proteins from cancer cell lines. This project focuses on D283 med and U-118mg cell lines which contribute to 12 missing protein identification.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-21 | MSV000087678 | MassIVE
Meiotic prophase I (MPI) is the most important event in mammalian meiosis. Even though MPI-related gene expression has been extensively investigated, the status of the chromosome binding proteins (CBPs) and the corresponding complexes in this process has been not scrutinized yet. A quantitative prot...
ORGANISM(S): Mus Musculus 
2023-06-08 | PXD035062 |
2 g developing siliques were harvested from the pMYB5:MYB5-GFP/myb5-2 complementary plants treated with 100 μM GA3 or 50 μM PAC. The 35S:GFP transgenic plants served as a negative control. Siliques were immediately crosslinked with 1% paraformaldehyde for 30 min on ice in a vacuum chamber. Total pro...
ORGANISM(S): Arabidopsis Thaliana 
2025-03-27 | PXD062288 |
The outbreak of coronavirus disease 2019 (COVID-19) is a global health emergency. Various omics results have been reported for COVID-19, but the molecular hallmarks of COVID-19, especially in those patients without comorbidities, have not been fully investigated. Here we collect blood samples from 2...
2021-10-06 | MTBLS2542 | MetaboLights
Histidyl dipeptides, such as carnosine, present in a micro-millimolar ranges in the heart, are synthesized via the enzyme carnosine synthase (Carns). These dipeptides facilitate glycolysis by proton buffering, form conjugates with reactive aldehydes, such as acrolein, and attenuate ischemia and repe...
ORGANISM(S): Mus <genus> 
2021-12-25 | PXD030647 |
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