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To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were red...
ORGANISM(S): Zea Mays 
2021-07-16 | PXD027378 |

Cold stress negatively affects maize (Zea mays L.) growth, development and yield. Metabolic adjustments contribute to the adaptation of maize under cold stress. We show here that the transcription factor INDUCER OF CBF EXPRESSION 1 (ZmICE1) plays a prominent role in reprogramming amino a...

2022-10-31 | MTBLS4404 | MetaboLights
For the analysis of phosphorylation sites of OST1, 8 g pSuper:OST1-Myc plants materials, treated with 4oC for 0 or 2 h, were used for protein extraction and immunoprecipitated with anti-Myc agarose. Then OST1-Myc proteins were eluted with 0.1 M glycine solution (pH 2.8) and mixed with 1 M Tris solut...
ORGANISM(S): Arabidopsis Thaliana 
2020-11-16 | PXD022539 |
MTD project_description Here we present a LC-MS analysis to detected the phosphorylation of AtANN1 at Ser289.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-11-13 | PXD022035 | Pride
ADP array data, comprised of 2217 samples of Asian ancestry (excluding the Japanese population from ADP). Samples were genotyped on different Illumina or Affy platform.
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