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ProteomicsAccurate identification of proteogenomic characterization in Mycolicibacterium smegmatis MC2155 by 14N and 15N double-labeling mass spectra
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037081 |
ProteomicsAc-LysargiNase Helps Genome Reannotation of Mycolicibacterium smegmatis MC2 155
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037080 |
UnknownThe human mitochondrial 12S rRNA m4C methyltransferase METTL15 is required for mitochondrial function.
Not available
S-EPMC7307190 | biostudies-literature
ProteomicsAn engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput screens
Coukos R, Yao D, Sanchez MI, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY. 2021. The trafficking of specific protein cohorts to the correct subcellular location at the correct time is essential for every signaling and regulatory process in biology. Gene perturbation scree...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-06 | MSV000087769 | MassIVE
MetabolomicsThe maturity in fetal pigs using a multi-fluid metabolomic approach
In mammalian species, the first days after birth are an important period for survival and the rates of mortality before weaning are high. In pigs, the perinatal deaths average 20% of the litter, with important economic and societal consequences. Among the factors influencing piglet survival at birth...
2020-07-07 | MTBLS1541 | MetaboLights
UnknownExclusive or Partial Breastfeeding for 6 Months Is Associated With Reduced Milk Sensitization and Risk of Eczema in Early Childhood: The PATCH Birth Cohort Study.
Not available
S-EPMC4839855 | biostudies-literature
UnknownEpigenome-Wide Meta-Analysis of Methylation in Children Related to Prenatal NO2 Air Pollution Exposure.
Not available
S-EPMC5226705 | biostudies-literature
ProteomicsNardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer
Pancreatic ductal adenocarcinoma (PDAC) develops through step-wise genetic and molecular alterations including Kras mutation and inactivation of apoptotic pathways. Here, we find that development of anoikis resistance and metastasis of KrasG12D-driven PDAC in mice is accelerated by deleting Plk3, ex...
ORGANISM(S): Homo sapiens (Human) 
2023-12-23 | PXD042993 | Pride
Plk3 Nardilysin (nrdc) metalloendopeptidase cleavage C-fos Anoikis Pancreatic ductal adenocarcinoma (pdac) Post-translational mechanism (ptm) Genetically engineered mouse models (gemms) Plk1 Kinase activation Metastasis 
MetabolomicsMetabolic phenotyping of urine from a dementia research cohort
To better understand the molecular mechanisms underpinning physiological variation in human populations, metabolic phenotyping approaches are increasingly being applied to studies involving hundreds and thousands of biofluid samples. Hyphenated ultra-performance liquid chromatography-mass spectromet...
2022-03-01 | MTBLS719 | MetaboLights
UnknownSynergistic action of specialized metabolites from divergent biosynthesis in the human oral microbiome.
Not available
S-EPMC12403116 | biostudies-literature
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