In this research, we investigated the molecular mechanisms and signaling pathways of Formula Q attenuating PCOS using a Tandem Mass Tag (TMT) proteomics approach, bioinformatics analyses and molecular biological technology. This study aimed to comprehensively characterize the multiple molecular mech...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-s...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
To explore the underlying mechanism by which OTUD6A induces PCa progression, we identified its binding proteins with an immunoprecipitation (IP)-mass spectrometry (MS) approach in two CRPC cell lines.
