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TranscriptomicsGenome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
ORGANISM(S): Saccharomyces cerevisiae 
2005-10-15 | E-GEOD-3456 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with calcium, sodium
In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-30 | E-SMDB-15 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with NaCl and FK506 vs NaCl
Cells were cultured with or without FK506 treatment and exposed to 0.8 M NaCl. Samples were collected from the drug-treated and control cultures at 30 and 45 min after Na+ addition. For the publication, the experiment was repeated twice, and the corresponding time points from each experiment were ...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-13 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with NaCl time series
Cells were grown in YPD medium (pH 5.0) at 30oC to a density of 0.6 X 107 cells/ml (A600=0.6). The culture was supplemented with ET at 30 oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing ET solution and 1.6 M NaCl was added to the ET...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-12 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated withNaCl + FK506, time series
Cells were grown in YPD medium (pH 5.0) at 30oC to a density of 0.6 X 107 cells/ml (A600=0.6). The culture was supplemented with FK506 (1.0 mg/ml) in ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing FK506 (1.0 mg/ml) and 1.6 ...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-14 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast wild type strain DBY7286 and crz1D strain YHY804 exposed to CaCl2
Wild-type strain DBY7286 and the crz1 mutant strain YHY804 were cultured and exposed to NaCl. Samples were collected at 30 and 45 min after Na+ addition. For the publication the experiment was repeated twice, and corresponding timepoints from each experiment were averaged. The first two arrays an...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-11 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with calcium + FK506 time series
Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with FK506 (1.0 mg/ml final concentration) in ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next,...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-30 | E-SMDB-8 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast wild type strain DBY7286 and crz1D strain YHY804 exposed to CaCl2
Wild-type strain DBY7286 and the crz1D strain YHY804 were cultured and exposed to CaCl2 as described for Experiments 1 and 2. Samples were collected at 15 and 30 min after Ca2+ addition. For the publication, the experiment was repeated twice, and corresponding timepoints were averaged between the e...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-10 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast ET/Calcium Time Series
Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) ...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-7 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with calcium + FK506 vs calcium
Cells were cultured with or without FK506 treatment and exposed to CaCl2. Samples were collected from the drug-treated and control cultures at 15 and 30 min after Ca2+ addition. For the publication the experiment was repeated twice, and corresponding time-points were averaged. The first two and t...
ORGANISM(S): Saccharomyces cerevisiae 
2005-01-01 | E-SMDB-9 | biostudies-arrayexpress
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