The present study had a total of 125 primary prostate cancer samples collected with paired non-tumor adjacent lung tissues from the treatment-naïve Chinese patients and proteogenomic analysis was performed.
In this project, SILAC-based quantitative proteomics approach was applied for phosphoproteomics analysis. The light samples were treated with ddH2O as control group, and the medium samples labeled by 2D2 of lysine (K4) and 13C6 of arginine (R6) were treated with 2DG, while the heavy samples labeled ...
We integrated quantitative proteomics and activity-based protein profiling (ABPP) approach to systematically elucidate the involved pathways and the covalent targets of natural products.