We used a deuterium-labeled aspirin (D-aspirin) together with mass spectrometry-based acetylomic analysis, termed “DAcMS”, to investigate the landscape of protein acetylation induced by aspirin. And we further demonstrated that aspirin acetylates proteins through both acetyl-CoA-dependent and -indep...
In this study, we investigated the impact of metformin on the cellular ubiquitinome and associated protein turnover. Through an integrated analysis combining ubiquitinome profiling with pulsed metabolic labeling, we found that metformin markedly suppresses global protein ubiquitination, including va...
Aging is characterized by a gradual decline in protein homeostasis. However, how protein dynamics changes with normal aging and in disease is less well understood. Here, we systemically characterize age-modulated proteome in Drosophila, from head and muscle tissues of somatic cells, and the testis a...
In the present study, we systematically profiled proteomes and N-glycoproteomes of cancerous, paracancerous, and distal noncancerous tissues from patients with breast cancer by DIA.
Protein turnover, the result of protein synthesis and degradation, is an important mechanism to preserve cellular integrity. The majority of cellular proteins are broken down over a period of hours to weeks to prevent a buildup of proteins that lose their function due to damage caused by misfolding,...
To characterize the accessibility of α-syn to LPC and DOPS, we performed cross-linking experiments coupled with mass spectrometry (XL-MS). We mixed 15N-labeled and unlabeled Ac-α-syn at a 1:1 ratio and selected the cross-linked fragments containing both 15N-labeled and unlabeled Ac-α-syn to map the ...
Our data describes the first atlas of long-lived proteins in somatic and reproductive tissues of Drosophila during adult lifespan, and reveals a preferential ubiquitylation in the aging proteome.
Creatine kinase (CK) is an essential metabolic enzyme mediating creatine/phosphocreatine interconversion and shuttle to replenish ATP for energy needs. Ablation of CK causes deficiency in energy supply that eventually results in reduced muscle burst activity and neurological disorders in mice. Besid...
