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Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on...

2024-05-22 | MTBLS2347 | MetaboLights
MicroRNAs (miRNAs) are small non-coding regulatory RNAs that play key roles in many diverse biological processes such as spermatogenesis. However, no study has been performed on the miRNA transcriptome of developing porcine testes. Here, we employed Solexa deep sequencing technology to extend the re...
ORGANISM(S): Sus scrofa 
XL-MS mass spectrometry analysis Cross-linked peptides were resolved for mass spectrometry analysis using a Dionex UltiMate 3000 RSLnano liquid chromatography system. Peptides were initially loaded from the autosampler onto an Acclaim PepMap 100 C18 LC Trap Cartridge (0.3 mm inside diameter, 5 mm le...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-10 | MSV000087174 | MassIVE
Ulcerative colitis (UC) is an inflammatory bowel disease characterized by disruption of the epithelial barrier, and its etiology has long been enigmatic. We here observed a significant depletion of macrophages directly beneath the UC epithelial layer, and hypothesized that this depletion may be caus...
ORGANISM(S): Aeromonas veronii bv. sobria 
2026-03-17 | PXD050471 | Pride
Succinate dehydrogenase, which is known as mitochondrial complex II, has proven to be a fascinating machinery, attracting renewed and increased interest in its involvement in human diseases. Herein, we find that succinate dehydrogenase assembly factor 4 (SDHAF4) is downregulated in cardiac muscle in...
2022-08-12 | MTBLS4579 | MetaboLights
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084679 | MassIVE
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