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ProteomicsThe RNF214-TEAD-YAP signaling axis promotes hepatocellular carcinoma progression via TEAD ubiquitylation
We established an APEX proximity labeling strategy in coupled with mass spectrometry to identify interacting proteins of RNF214. In this approach, we first fused an engineered ascorbate peroxidase (APEX2) to either N-terminus or C-terminus of RNF214, expressed these two fusion proteins in HLF, an HC...
ORGANISM(S): Homo Sapiens 
2024-02-18 | PXD049453 |
Proteomicsthe proteomics of IGFBP6 in endothelial cells (HUVECs)
To unravel the direct mechanism of the anti-inflammatory effects of IGFBP6 in ECs, we analysed HUVECs treated with either adenovirus-Flag-IGFBP6 (Ad-IGFBP6) or control adenovirus (Ad-NC). We precipitated IGFBP6 from overexpressed ECs followed by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2024-05-13 | PXD052204 |
UnknownEssential Oils of Five Baccharis Species: Investigations on the Chemical Composition and Biological Activities.
Not available
S-EPMC6222634 | biostudies-literature
MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
ProteomicsCREB3 suppresses hepatocellular carcinoma progression by depressing AKT signaling through competitively binding with INSR and transcriptionally activating RBM38
CREB3 is suggested to interact with many proteins. We explored the potential binding proteins using co-IP.
ORGANISM(S): Homo Sapiens 
2024-05-27 | PXD052604 |
ProteomicsMouse Testis IP-MS Analysis of TEX44 Interacting Proteins
Through whole-exome sequencing of 800 patients with asthenozoospermia, we identified biallelic TEX44 variants in six individuals, all of whom exhibited defective mitochondrial sheath assembly and impaired sperm motility. Using Tex44 knockout mice, we demonstrated that TEX44 interacts with carnitine ...
ORGANISM(S): Mus Musculus 
2025-02-20 | PXD060989 |
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