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ProteomicsLRRC71 is essential for sperm motility, fertilization and male fertility
Although sperm supply half the genomic material necessary for generating viable offspring in sexual reproduction, the core molecular mechanisms underlying their formation remain largely unelucidated. Here, we demonstrate that leucine-rich repeat-containing 71 (LRRC71) is essential for spermatogenesi...
ORGANISM(S): Mus Musculus 
2026-04-29 | PXD077837 |
TranscriptomicsRegulation of alternative cleavage and polyadenylation by 3’ end processing and splicing factors
Alternative cleavage and polyadenylation (APA) results in mRNA isoforms containing different 3’ untranslated regions (3’UTRs) and/or coding sequences. How core cleavage and polyadenylation (C/P) factors regulate APA is not well understood. Using siRNA knockdown coupled with deep sequencing, we found...
ORGANISM(S): Mus musculus 
2015-04-01 | E-GEOD-62001 | biostudies-arrayexpress
TranscriptomicsAnalysis of alternative cleavage and polyadenylation by 3' region extraction and deep sequencing
Alternative cleavage and polyadenylation (APA) generates diverse mRNA isoforms. We developed 3' region extraction and deep sequencing (3'READS) to address mispriming issues that commonly plague poly(A) site (pA) identification, and we used the method to comprehensively map pAs in the mouse genome. T...
ORGANISM(S): Mus musculus 
2012-12-17 | E-GEOD-42398 | biostudies-arrayexpress
Proteomicsdichotomic size-exclusion chromatography（SEC）
directDIA proteomics raw data of plasma extracellular vesicles isolated by SEC and UC
ORGANISM(S): Homo Sapiens 
2021-05-17 | PXD026034 |
ProteomicsKAT8-TurboID labelling proteins
V5-TurboID-KAT8-expressing A375 stable cells were treated with biotin and then underwent streptavidin purification and mass spectrometry.
ORGANISM(S): Homo Sapiens 
2022-12-05 | PXD038565 |
ProteomicsIRF1 acetylation sites by KAT8
Aim to identify the IRF1 acetylation site by KAT8 in cotransfected HEK293T cells
ORGANISM(S): Homo Sapiens 
2022-12-05 | PXD038568 |
ProteomicsThe proteome data of human mesenchymal stem cell derived from healthy donors
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
2023-06-12 | PXD033812 |
ProteomicsThe proteome data of extracellular vesicles derived from human umbilical cord and bone marrow mesenchymal stem cells
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
2023-06-12 | PXD042977 |
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