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Following DPST crosslinking of human neuroblastoma cells, nuclear-cytoplasmic fractionation was performed using hypotonic lysis to obtain cytoplasmic and nuclear protein compartments. Through systematic analysis of crosslinking mass spectrometry data, we elucidated how subcellular compartmentalized ...
ORGANISM(S): Homo Sapiens 
2025-04-14 | PXD062913 |
Comprehensive interactome analysis of targeted proteins is of great significance to understand how pro-teins work together for functional regulation. Until now, affinity purification strategy with mass spec-trometry (AP-MS) has been recognized as the most widely used technique for studying protein-p...
ORGANISM(S): Homo Sapiens 
2022-12-01 | PXD038473 |
Amyloid deposits are key pathological features of neurodegenerative diseases, yet their structural heterogeneity coupled with a stable β-sheet fibrillar architecture makes them extremely insoluble and difficult to isolate. Through systematic screening and molecular dynamics simulations, we identifie...
ORGANISM(S): Mus Musculus 
2025-10-17 | PXD069689 |
Here, we presented a streamlined workflow to measure abundant AMG510 modified peptides including that of KRAS/G12C by direct profiling, and a pan-AMG510 antibody peptide IP workflow to profile less abundant AMG510 off-targets.
ORGANISM(S): Homo Sapiens 
2023-01-11 | PXD029449 |
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