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TranscriptomicsGenome-wide analysis of the BR response in msg2 and axr2
Although a wide range of interactions between BRs and auxin have been recognized, knowledge about the direct molecular mechanism of interaction between them in specific physiological processes is very limited. In this study we found that auxin resisitent mutant msg2/iaa19 and arf7 were also resisit...
ORGANISM(S): Arabidopsis thaliana 
2012-12-16 | E-GEOD-33412 | biostudies-arrayexpress
TranscriptomicsExpression data from early rice endosperm
We used microarrays to detail the global programme of gene expression underlying cellularization and identified distinct classes of regulated genes during this process. Rice endosperm at 2 days after pollination(DAP) was selected for RNA extraction and hybridization on Affymetrix microarrays. We sou...
ORGANISM(S): Oryza sativa 
2015-09-01 | E-GEOD-57615 | biostudies-arrayexpress
GenomicsGenome-wide maps of DNA methylation state in rice embryo and endosperm
We report the application of methylacytosine immunoprecipetation combined with Illumina sequencing (MeDIP-seq) for high-throughput profiling of DNA methylation in rice embryo 3, 6, 9 DAP and endosperm 2, 3, 6, 9 DAP. A total number of 12-14 million of 2×49 bp paired-end reads was generated for each ...
ORGANISM(S): Oryza sativa 
2015-09-01 | E-GEOD-57706 | biostudies-arrayexpress
UnknownCatalogue of fungi in China 3. New taxa of macrofungi from southern Xizang, China.
Not available
S-EPMC11899225 | biostudies-literature
UnknownSystematic profiling of SARS-CoV-2-specific IgG responses elicited by an inactivated virus vaccine identifies peptides and proteins for predicting vaccination efficacy.
Not available
S-EPMC8367966 | biostudies-literature
ProteomicsMass spectrometry to exam noval receptors of the The Omicron Variant of SARS-CoV-2 RBD captured by SPIDER
We used SPIDER to examine the cell-surface receptors for specific ligands while these receptors were in their native membranes within intact cells in culture. Here we coupled the SPIDER assay with biotinylated RBD of the Omicron Variant of SARS-CoV-2 and biotinylated RBD of SARS-CoV-2. Then we perfo...
ORGANISM(S): Homo Sapiens Chlorocebus Sabaeus 
2022-01-18 | PXD031035 |
ProteomicsPupylation sites on protein GFP-PupE
Characterization of GFP-Pup after Pupylation assays by mass spectrometry.
ORGANISM(S): Mycobacterium Tuberculosis H37rv_2009 
2021-06-04 | PXD026478 |
ProteomicsCheA pupylation sites identified by SPIDER assay
We used CheAs and biotin-CheZ to validate the efficiency of SPIDER capturing protein-protein interaction. Pupylation sites on WT CheAs after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e.,...
ORGANISM(S): Escherichia Coli 
2021-06-04 | PXD026509 |
ProteomicsCobB interacting proteins identified by SPIDER assay
To assess whether SPIDER could detect transient interactions such as enzymes and their substrates, we examined the interactome of the E. coli protein deacetylase CobB. As the only member of the Sir2 family of deacetylases in E. coli, CobB is known to play a role in many different pathways but their ...
ORGANISM(S): Escherichia Coli 
2021-06-04 | PXD026514 |
ProteomicsPupylation sites on protein Sox2
As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an ...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026517 |
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