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ProteomicsNative mass spectrometry of an allosteric heterodimer of SARS-CoV-2
The capability of native MS to probe protein-protein and protein-ligand interactions was exemplified with the allosteric heterodimer from SARS-CoV-2 consisting of the nonstructural proteins (nsp) nsp10 and nsp16. Complex dynamics, allostery, and ligand binding were shown. A metadata Excel file, 'nsp...
ORGANISM(S): Escherichia Coli Bl21(de3) (ncbitaxon:469008) 
2023-08-28 | MSV000092776 | MassIVE
ProteomicsShotgun proteomics of soybean root nodules infected by Bradyrhizobium japonicum
Global bottom-up proteomics analysis of proteins purified from soybean root nodules infected with either WT or nifH- mutant Bradyrhizobium japonicum. Nine glycoproteins containing Lewis-a N-glycans, with 3 distinct Lewis-a epitopes (Hex:5 HexNAc:4 dHex:3 Pent:1, Hex:4 HexNAc:4 dHex:2 Pent:1, and Hex...
ORGANISM(S): Glycine Max (ncbitaxon:3847) 
2022-01-31 | MSV000088754 | MassIVE
ProteomicsStructure elucidation for tunable hetero-assembly of a plant PDX1.2/PDX1.3 pseudoenzyme-enzyme pair
Mass spectrometry raw data including peptide mapping, and top down liquid chromatography mass spectrometry for Arabidopsis PDX1.2/PDX1.3 homo- and hetero-complexes produced using cell-free expression. Purified proteins from cell-free expression were digested using trifluoroethanol protocol for pepti...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-04-06 | MSV000085233 | MassIVE
ProteomicsSpatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
We demonstrate spatially resolved top-down proteomics (TDP) for proteoform identification and quantification from small sections of rat brain. We used the nanoPOTS (nanodroplet Processing in One pot for Trace Samples) platform, further improving the sample preparation by adding benzonase in the extr...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) Homo Sapiens (ncbitaxon:9606) 
2022-03-30 | MSV000089163 | MassIVE
ProteomicsOnline Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of SARS-CoV-2 Spike Receptor Binding Domain
Online LCMS intact glycoform separation of SARS-CoV-2 spike receptor binding domain proteins expressed in HEK-293 cells from the vendors Sino Biological or RayBiotech. C2 RPLC, CZE, and HILIC separation methods are compared as well as top-down and bottom-up analysis to define RBD glycosylation patte...
ORGANISM(S): Sars Coronavirus (ncbitaxon:227859) Homo Sapiens (ncbitaxon:9606) 
2021-12-21 | MSV000088601 | MassIVE
ProteomicsTop-down proteomic analysis with FAIMS of Alzheimer's disease derived brain tissue
Proteomic datasets collected from a single human midfrontal cortex tissue sample. Tissue was homogenized in 8 M Urea, 10 mM ABC, and 10 mM TCEP before subsequent cleanup with 100K and 3K MWCO filters. NanoLC was performed for RP separation. Three technical replicates were collected without FAIMS. Se...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-14 | MSV000086696 | MassIVE
ProteomicsIdentification of enriched O-GlcNAc proteins in hypertrophic mouse hearts
We used C57/Bl6 mice subjected to sham or transverse aortic constriction (TAC) to create pressure-overload hypertrophy (POH). From the hearts, we stabilized and labelled the O-GlcNAc moiety with tetramethylrhodamine azide (TAMRA) before enriching by TAMRA immunoprecipitation (files with "TAMRAIP"). ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-02-07 | MSV000088797 | MassIVE
ProteomicsIdentification of enriched O-GlcNAc proteins in hypertrophic and normal mouse hearts
Bottom up proteomics of O-GlcNAc antibody enriched proteins from mouse heart tissue (hypertrophic vs. normal). O-GlcNAc proteins were labeled with a click chemistry kit with the tetramethylrhodamine (TAMRA) tag, then the labeled proteins were enriched with TAMRA antibody for proteomics experiments.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-12-06 | MSV000088525 | MassIVE
ProteomicsDifferential solubilization and top-down proteomics with FAIMS control experiments applied to mouse brain
5-35 mg brain tissue plugs from mouse left or right hemisphere were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homogenization buffer (H...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-12-25 | MSV000093727 | MassIVE
ProteomicsLarge scale, quantitative top-down proteomic analysis of human frontal gyrus brain tissue samples in the context of Alzheimer's disease
15-35 mg brain tissue plugs derived from dorsal lateral prefrontal cortex (DLPFC) were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homog...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-26 | MSV000093728 | MassIVE
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