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ProteomicsCD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
Serum Immunoaffinity isolation Cd9 Extracellular vesicles Hplc Proteomics 
ProteomicsGlycopeptide Biomarkers for HCC Detection in Patients with NASH
A comprehensive screening of site-specific N-glycopeptides in serum haptoglobin (Hp), a reporter molecule for aberrant glycosylation in HCC, has been performed to characterize glycopeptide markers for NASH-related HCCs.
ORGANISM(S): Homo sapiens (Human) 
2020-05-22 | PXD018502 | Pride
Human Serum Glycopeptide Lc-ethcd-ms/ms 
ProteomicsIntact N-glycopeptides in Serum Haptoglobin between HCC and Cirrhosis
A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD011239 | Pride
Human Serum Haptoglobin Lc-ethcd-ms/ms Liver 
ProteomicsmHtt tobacco roots proteome, QEHFX
Samples were analyzed using a nanoACQUITY UPLC system (Waters) coupled to a Q-Exactive HF-X high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly the sample was first trapped on a Symmetry C18 180 micron x 20 mm trapping column (5 microlite...
ORGANISM(S): Nicotiana Tabacum (ncbitaxon:4097) 
2019-11-07 | MSV000084557 | MassIVE
MetabolomicsIntegrated multi-omics analysis and experimental verification reveal the involvement of the PI3K/Akt signaling pathway in myometrial fibrosis of adenomyosis.

This analysis established comprehensive metabolomic profiles of adenomyosis patients and revealed widespread alterations in the metabolome within normal and fibrotic myometrium. Integrated analysis shown significant associations between metabolomic data and fibrosis . This study is the first to d...

2025-05-12 | MTBLS11965 | MetaboLights
ProteomicsThe SUMOylated proteins interact with the DNA of porcine circovirus type 2
The PCV2 DNA pulled-down proteins were further immunoprecipitated with an anti-Pan SUMO antibody, and the precipitated proteins were analyzed by Liquid Chromatography-Mass Spectrometer/Mass Spectrometry
ORGANISM(S): Sus Scrofa Domesticus 
2023-08-23 | PXD044762 |
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