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ProteomicsComparative analysis of mRNA degradation and protein degradation in prostate tissues indicates high stability of proteins
To benchmark the PIN algorithm that quantifies the extent of protein degradation in samples, we generated a set of “ground truth” samples, in which the levels of proteome degradation were known and independently validated by western blotting. Protein extracts of HeLa Kyoto cells were treated with th...
ORGANISM(S): Homo sapiens (Human) 
2019-06-12 | PXD013622 | Pride
Benchmarking  proteome integrity 
ProteomicsComparative analysis of mRNA degradation and protein degradation in 68 pairs of adjacent prostate tissue samples indicates high stability of proteins
Here we investigated the degradation of mRNA and protein in 68 pairs of adjacent prostate tissue samples using RNA-seq and pressure cycling technology (PCT) coupled with SWATH mass spectrometry and developed a score, the Proteome Integrity Number (PIN), to quantify the extent of protein degradation ...
ORGANISM(S): Homo sapiens (Human) 
2019-06-12 | PXD007841 | Pride
Dia/swath  clinical tissues  protein stability 
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