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To monitor changes to cellular and viral SUMO-modified proteins during EBV reactivation. SUMO1 and SUMO2 conjugates were studied using KGG mutant forms of SUMO and GG-K specific antibody enrichment of SUMO modified peptides. Time-points of 12h and 24h post-reactivation were monitored. Total cellular...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD034631 | Pride
Bladder outlet obstruction (BOO) causes lower urinary tract symptoms and objectifiable urodynamic changes in bladder function. We carried out an integrated transcriptome and proteome analysis of bladder samples from male patients with BOO before and 3 months after de-obstruction surgery (transurethr...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD044777 | Pride
DNA methylation is an epigenetic modification associated with transcriptional repression of promoters and is essential for mammalian development. Establishment of DNA methylation is mediated by the de novo DNA methyltransferases DNMT3A and DNMT3B, whereas DNMT1 ensures maintenance of methylation thr...
ORGANISM(S): Mus musculus 
In this work, we used our recently developed method Spec-seq to characterize the binding specificity of Glucocorticoid receptor in vitro. This GR Spec-seq experiment has been run twice separately (Dec 2014 and Mar 2015) with different library compositions. The basic workflow is the same as our previ...
ORGANISM(S): Homo sapiens 
This SuperSeries is composed of the SubSeries listed below. DNA methylation is an epigenetic modification associated with transcriptional repression of promoters and is essential for mammalian development. Establishment of DNA methylation is mediated by the de novo DNA methyltransferases DNMT3A and...
ORGANISM(S): Mus musculus 
Next Generation Sequencing technologies have enabled de novo gene fusion discovery that could reveal candidates with therapeutic significance in cancer. Here we present an open-source software package, ChimeraScan, for the discovery of chimeric transcription between two independent transcripts. Thre...
ORGANISM(S): Homo sapiens 
Tumours induce de novo steroidogenesis in immune cells. We wanted to define the gene expression identity of intratumoural steroidogenic immune cells and compare them with the transcriptome with non-steroidogenic cells. Cyp11a1 expression is a faithful biomarker of de novo steroidogenesis (i.e. Cyp11...
ORGANISM(S): Mus musculus 
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