High-speed live cell interferometry (HSLCI) images of bioprinted leiomyosarcoma patient-derived tumor organoids

ABSTRACT: We present a protocol to bioprint cells within a temperature-sensitive ECM in thin, flat, square-shaped patterns in 96-well plates to establish three-dimensional (3D) cultures for efficient, high throughput, time-resolved quantitative phase imaging (QPI) at single-organoid resolution. QPI of 3D-bioprinted organoids using high-speed live cell interferometry (HSLCI) coupled with machine learning analyses enables label-free quantification of biomass, growth kinetics, and drug response profiles of thousands of individual organoids per experiment. We demonstrate that the protocol can be leveraged to automatically generate plates containing thousands of organoids for high throughput imaging and drug screening experiments, quantify growth and drug response heterogeneity, resolve rare phenotypes, and identify predictive features of drug response profiles for fundamental studies and therapeutic decision-making. The imaging files uploaded are time-series phase images for each imaging position stored in .mat files.

SUBMITTER: Bowen Wang 

PROVIDER: S-BIAD3391 | bioimages |

REPOSITORIES: bioimages