Transcription profiling of rat Myasthenia gravis model, diaphragm, extensor digitorum longus and extraocular muscles

ABSTRACT: gene expression profile in diaphragm (DIA), extensor digitorum longus (EDL) and extraocular (EOM) muscles of rats with actively induced experimentally acquired MG (EAMG) using Affymetrix rat RAE230 gene chip. Experiment Overall Design: Total RNA was obtained with TRIzol (Invitrogen Carlsbad, CA) following the manufacturers recommended protocol. Tissues from 3 animals were combined into each RNA sample to decrease inter-subject variability. RNA pellets were cleaned by RNasey kits and resuspended at 1 μg RNA/μl DEPC-treated water and 5 ug was used in a reverse transcription reaction (SuperScript II; Life Technologies, Rockville, MD) to generate first strand cDNA. Double strand cDNA was synthesized and used in an in vitro transcription (IVT) reaction to generate biotinylated cRNA. Fragmen

ORGANISM(S): Rattus norvegicus

SUBMITTER: Bendi Gong 

PROVIDER: E-GEOD-11465 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress