Sanguinarine, putative anticancer therapeutic, interacts,modulates and transcripts in the context of chromatin

ABSTRACT: DNA binding anticancer agents cause alteration in chromatin structure and dynamics. Here, we report the dynamic interaction of the DNA intercalator and potential anticancer, plant alkaloid, Sanguinarine (SGR) with chromatin. Association of SGR with different levels of chromatin structure was found to be enthalpydriven. Apart from DNA it binds with comparable affinity to histones and induces chromatin aggregation. The dual binding property of SGR leads to inhibition of core histone modifications. Although, it potently inhibits H3K9 methylation by G9a in vitro, H3K4 and H3R17 methylation are more profoundly inhibited in cells. SGR inhibits histone acetylation both in vitro and in vivo. It does not affect the in vitro transcription from DNA template but significantly represses acetylation dep

ORGANISM(S): Homo sapiens

SUBMITTER: Tapas Kundu 

PROVIDER: E-GEOD-14314 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress