Cellulose-induced regulation of Phanaerochaete chrysosporium genes

ABSTRACT: Transcript profiles of Phanerochaete chrysosporium grown on different substrates were analyzed. Array design based on the DoE's Joint Genome Institute's v2.1 annotation. Goal is to define genes involved in cellulose degradation. Keywords: gene expression under different culture conditions From a data set of 10,004 unique alleles, each Roche NimbleGen (Madison, WI) array featured 12 unique 60mer probes per gene, all in triplicate. (Seven gene models composed mostly of repetitive DNA were represented by only 2 to 11 60mers.) Total RNA was purified from below mentioned media. In short, cultures were harvested by filtering through Miracloth (Calbiochem, EMD Biosciences, Gibbstown, NJ), squeeze dried and snap frozen in liquid nitrogen. Pellets were stored at -80 C until use. Extraction buffer

ORGANISM(S): Phanerochaete chrysosporium

SUBMITTER: Dan Cullen 

PROVIDER: E-GEOD-14734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress