Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

66 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Study on the genomic changes in ColIa2 Cre x RBP-Jkappa loxp/loxp mice

ABSTRACT: Skin tumor formed in the ColIa2 Cre x RBP-Jkappa loxp/loxp mice was analyzed for genomic changes by comparing with normal tissues from the same mice. Genomic DNA were isolated from each individual tumor and compared with the DNA extracted from the brain tissue in the same mouse.

ORGANISM(S): Mus musculus

SUBMITTER: Bing Hu

PROVIDER: E-GEOD-36464 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Study on the genomic changes in ColIa2 Cre x RBP-Jkappa loxp/loxp mice

Project description:Skin tumor formed in the ColIa2 Cre x RBP-Jkappa loxp/loxp mice was analyzed for genomic changes by comparing with normal tissues from the same mice.

2012-05-01 | GSE36464 | GEO

QKI's function in neural stem cells (NSCs) and pre-malignant NSCs (PM-NSCs) and transcriptome of Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP (QPP) mouse glioblastoma

Project description:This study is to determine the impact of QKI deletion on transcriptomes of mouse NSC and PM-NSC and to analyze the transcriptomic profiles of Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP (QPP) mouse glioblastoma and to determine which subtypes these tumors belong to

2016-11-10 | GSE79889 | GEO

Rbp

Project description:Drosophila melanogaster Rbp, RIM-binding protein, is differentially expressed in 8 experiment(s);

| FBgn0262483 | ExpressionAtlas

Rbp

Project description:Drosophila melanogaster Rbp, RIM-binding protein, is expressed in 3 baseline experiment(s);

| FBgn0262483 | ExpressionAtlas

RNA Sequencing of mouse E15.5 Six2-Cre Irx3/5(loxP/loxP) whole kidneys

Project description:RNA Sequencing of mouse E15.5 Six2-Cre Irx3/5(loxP/loxP) whole kidneys

| PRJNA1134083 | ENA

Genome-wide transcriptional regulation by RBP-J

Project description:We sequenced mRNAs from bone borrow derived macrophages derived from the control (WT) and RBP-J conditional knockout mice (RBP-J KO; Rbpj f/f;LysM cre).

2016-12-01 | GSE53218 | GEO

Engineering spacer specificity of the Cre/loxP system

Project description:Translational research on the Cre/loxP recombination system focuses on enhancing its specificity by modifying Cre/DNA interactions. Despite extensive efforts, the exact mechanisms governing how Cre distinguishes between substrates remains elusive. Cre recognizes 13 bp inverted repeats, initiating recombination in the 8 bp spacer region. While literature suggests that efficient recombination proceeds between lox sites with non-loxP spacer sequences when both lox sites have matching spacers, experimental validation for this assumption is lacking. To fill this gap, we investigated target site variations of identical pairs of the loxP 8 bp spacer region, screening 6,000 unique loxP-like sequences. Approximately 84% of these sites exhibited efficient recombination, affirming the flexibility of spacer sequences for catalysis. However, certain spacers negatively impacted recombination, emphasizing sequence dependence. Directed evolution of Cre on inefficiently recombined spacers not only yielded recombinases with enhanced activity but also mutants with reprogrammed selective activity. Mutations altering spacer specificity were identified, and molecular modelling and dynamics simulations elucidated the mechanism behind this specificity switch. These findings highlight the potential to fine-tune site-specific recombinases for spacer sequence specificity, offering a novel concept to enhance the applied properties of designer-recombinases for genome engineering applications.

2024-07-25 | GSE254392 | GEO

Genome-wide microRNA expression in WT and RBP-J KO BMDMs

Project description:RBP-J is a master transcriptional factor of Notch signaling, which plays important roles in developmental processes as well as regulating macrophage-mediated inflammatory responses. However, the regulation of RBP-J on miRNAs are less studied. So we sequenced microRNAs from BMDMs derived from the LyZ2 Cre control (WT) and RBP-J conditional knockout mice (RBP-J KO; Rbpjf/f LyZ2 Cre) to address the roles of RBP-J on regulating miRNAs in macrophages.

2019-12-11 | GSE103220 | GEO

Transcriptome changes caused by RBP-L knockdown in rice grains

Project description:A T-DNA insertion within RBP-L 3’UTR resulted in 10-25% expression level of RBP-L gene compared to wild-type. The reduced expression of RBP-L caused partial mis-localization of glutelin and prolamine RNAs and conferred other general growth defects including dwarfism, late flowering and smaller seeds. Transcriptome analysis showed that RBP-L knockdown greatly affected the expression of prolamine family genes and many genes invovled in essential biological pathways during plant development.

2019-05-22 | GSE124301 | GEO

Cre-loxP-induced Ewsr1-Atf1 translocation tumors

Project description:Cre-loxP-induced Ewsr1-Atf1 translocation tumors

| PRJNA666346 | ENA