Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from sexual flocculation cells of S. pombe

ABSTRACT: The h+ and h- haploid cells of S. pombe mixtures flocculated and formed diploid cells when cultured in carbon limited YEPME medium. We first cultured S. pombe h+ YHL6381 and h- SPQ-01 cells respectively in YEPD medium and then mixed them at an 1:1 ratio and further cultured in YEPME medium. Sexual flocculation occured. We used microarrays to detail the the global programme of gene expression of flocculated YHL6381 and SPQ-01 mixtures cultured in YEPME, compared with the non flocculated cells mixtures cultured in YEPD, and identified distinct classes of up-regulated genes during this process. We first cultured S. pombe h+ YHL6381 and h- SPQ-01 cells respectively in YEPD medium and then mixed them at an 1:1 ratio and further cultured in a new medium for 90min. The flocculated cells mixtures cultured in YEPME named YSD, while the non flocculated cells mixtures cultured in YEPD named YSM.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Rongpeng Li

PROVIDER: E-GEOD-43250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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