Temporal gene expression across osteoblastogenesis.

ABSTRACT: Purpose: Osteoblast cells mature from a mesenchymal stem cell pool to become cells capable of forming bone matrix and mineralizing this matrix. The goal of this study was to characterize temporal changes in the transcriptome across osteoblast maturation, starting with committed mesenchymal stem cell/ early pre-osteoblast stage through to mature osteoblasts capable of matrix mineralization. Methods: Enriched populations of pre-osteoblast like cells were obtained from neonatal calvaria from C57BL/6J mice expressing CFP under the control of the Col3.6 promoter. These cells were placed into culture for 4 days, removed from culture and subjected FACS sorting based on the presence/absence of CFP expression. Cells expressing CFP were returned to culture, subjected to an osteoblast differentia

ORGANISM(S): Mus musculus

SUBMITTER: Cheryl Ackert-Bicknell 

PROVIDER: E-GEOD-54461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress