Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human fibrosarcoma cells to study the relative contributions of Jak1 and Jak2 in IFNg signaling

ABSTRACT: General impact of Jak1 and Jak2 inhibition on IFNg-mediated target gene expression. U4C-Jak1AS and g2A-Jak2AS cells were stimulated with IFNg and treated with either 1NM-PP1 (to inhibit the activity only of the analog-sensitive mutant) or JI1 (to suppress both wild-type and analog-sensitive Jaks) for 24h.

ORGANISM(S): Homo sapiens

SUBMITTER: Arnaud Muller

PROVIDER: E-MEXP-2621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Changes of miRNA expression in granulocytes of patients with polycythemia vera (PV) and essential thrombocythemia (ET) receiving INCB018424 in a Phase 2 study

Project description:A global microRNA expression profile was obtained from gradient-purified granulocytes (>95% pure) collected at the time of screening and at cycle 4 of treatment Protocol #18424-256 is a Phase 2 study of the JAK1 and JAK2 inhibitor INCB01842 in patients with advanced polycythemia vera (PV) and essential thrombocythemia (ET) refractory to hydroxyurea; The aim was to to determine whether treatment with INC180424 was associated with changes in the global microRNA expression profile we compared granulocytes collected at baseline (screening) and at cycle 4

2012-09-26 | E-GEOD-32194 | biostudies-arrayexpress

Transcription profiling of liver from mice deficient for IFNg or both IFNg and Suppressor of Cytokine Signalling-1 at 0.5 to 48 hours after INFg treatment

Project description:Timecourse analysis of Interferon-Gamma (IFNg) signalling in mice deficient for IFNg or both IFNg and Suppressor of Cytokine Signalling-1 (SOCS1).

2007-06-27 | E-GEOD-4232 | biostudies-arrayexpress

Transcription profiling time series of mouse bone marrow derived macrophages after interferon-gamma treatment

Project description:We have examined the transcriptional events in mouse bone marrow derived macrophages (MBDM) with interferon-gamma (Ifng)at 2, 4 & 8 h following treatment or pre-treatment (0 h).

2008-03-18 | E-MEXP-1490 | biostudies-arrayexpress

Succination analysis of JAK1 and IKK2 using targeted MS/MS analysis

Project description:Using Affinity Purification in tandem to targeted LC-MS/MS analysis, we identified possible succination sites of JAK1 and IKK2 proteins follwoing DMF treatement. We looked for the specific 2-Dimethylsuccinyl modifications of Cysteins residues.

2021-03-24 | PXD024441 | Pride

Transcription profiling of Listeria monocytogenes intracellularly replicating in mouse macrophages activated by IFN-gamma

Project description:The present study show the impact of macrophages activation by IFN-Gamma on the gene regulation in intracellularly replicating L. monocytogenes and provide an overview about additionally adaption mechanisms of L. monocytogenes to immune response.

2010-03-11 | E-TABM-663 | biostudies-arrayexpress

Transcription profiling of human HEL cells treated with either 5 uM of JI1 or 10 uM of Erlotinib reveal growth of leukemic Jak2-V617F positive cells is mutually suppressed by Jak inhibitors and Erlotinib through distinct mechanisms.

Project description:Gene expression profiling experiments were performed using the Agilent 44K whole human genome microarrays (Agilent Biotechnologies, Diegem, Belgium) according to the two-color gene expression analysis (Quick Amp labeling) protocol (version 5.7) from the manufacturer. Hybridizations were performed by comparing 300 ng of total RNA extracted from HEL cells treated with either 5 ?M of JI1 or 10 ?M of Erlotinib with that obtained from untreated cells. For each treatment, three biological replicates were analyzed in duplicate including a dye swap. Only high quality RNAs with a ribosomal RNA ratio greater than 1.9 and no evidence of degradation, as evaluated using the Agilent Bioanalyzer 2100 RNA 6000 nano assay, were used in this study. Microarray images were quantified using the GenePix Pro 6.1 software (Molecular Devices, Sunnyvale, CA, USA) as described in supplemental information.

2009-08-10 | E-MEXP-2244 | biostudies-arrayexpress

Bulk RNA-Seq to study the effects of CENP-A overexpression, p53 status, and X- irradiation treatment in MCF-10-2A cells

Project description:We performed bulk RNA-Seq to investigate global transcriptional changes upon overexpression of the centromeric H3 variant CenH3/CENP-A in p53 wild-type and defective cells, and after X-irradiation treatment. We established clonal MCF-10-2A TetOn-CENPA-FLAG-HA cell lines where CENP-A can be reversibly induced by Doxycycline (Dox) treatment. Upon CENP-A overexpression, these cells exhibit different radiosensitivity depending on p53 status. In order to profile global changes in expression, we compared MCF-10-2A TetOn-CENPA-FLAG-HA cells expressing either empty vector (p53-WT) or dominant-negative p53 (p53-DN) with 0X Dox (no Dox), 1X Dox (10 ng/ml), or 10X Dox (100 ng/ml) for 24h. At time 0, we irradiated one set of cells by X-ray generator (4gy) while a control set remained un-irradiated (0gy). 6h later, we extracted RNA for RNA-seq.

2021-03-01 | E-MTAB-9867 | biostudies-arrayexpress

Transcription profiling of mouse IFNg-primed C57BL/6 and C3H.BL6-sst1 bone-marrow macrophages infected with mycobacterium tuberculosis

Project description:Time-course transcriptional profiling of IFNg-primed C57/BL6 and C3H.BL6-sst1 bone-marrow macrophages infected with mycobacterium tuberculosis (MTB)

2010-01-07 | E-MTAB-90 | biostudies-arrayexpress

Transcription profiling of human normal progenitor cells mutated for RUNX1-RUNX1 during myeloid and erythroid development to identify genes disregulated by RUNX1-RUNX1

Project description:In order to identify genes dysregulated by the aberrant transcriptional activity of RUNX1-RUNX1T1, we used microarrays to determine the effect of this mutation on gene expression during myeloid and erythroid development of normal human progenitor cells.

2007-10-01 | E-MEXP-583 | biostudies-arrayexpress

Transcription profiling of mouse brain to identify age-related transcriptional changes and the effect of dietary supplementation of vitamin E

Project description:We established the transcriptional profile of brain aging and examine the global effects of vitamin E supplementation on age-related alterations in expression in the aged mouse brain. Experiment Overall Design: Gene expression profiles were obtained from the neocortex tissues of 5-month-old controls, 30-month-old controls and 30-month old B6C3F1 mice with middle age-onset supplementation of alpha-tocopherol or a mixture of alpha and gamma-tocopherol (500mg/kg of each tocopherol).

2009-09-23 | E-GEOD-8150 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data