Non-coding RNA-Seq of Listeria monocytogenes from bacteria growing extracellularly and in macrophages

ABSTRACT: Small non-coding RNAs (sRNAs) are widespread effectors of post-transcriptional gene regulation in bacteria. Currently extensive information exists on the sRNAs of Listeria monocytogenes (Lm) expressed during growth in extracellular environments. We used deep sequencing of cDNAs obtained from fractioned RNA (<500nt) isolated from extracellularly growing bacteria and from Lm-infected macrophages to catalog the sRNA repertoire during intracellular bacterial growth. Here we report on the discovery of 150 regulatory RNAs of which 71 have never been previously described. A total of 29 regulatory RNAs, including small non-coding antisense RNAs, are specifically expressed intracellularly. We validated highly expressed sRNAs by Northern blotting and demonstrated by the construction and characteriza

ORGANISM(S): Listeria monocytogenes EGD-e

SUBMITTER: Andre Billion 

PROVIDER: E-MTAB-329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress