Fat, lazy and indifferent - profiling persistent drug-tolerant tubercule bacilli from patient sputa during treatment predicts drug efficacy

ABSTRACT: Amplified M.tb RNA derived from 15 subjects at multiple time intervals before and during chemotherapy (totalling 52 samples) was profiled alongside M.tb H37Rv RNA extracted from in vitro log phase bacilli (2 biological replicates hybridised in duplicate) as a standardised comparator. Amplified mycobacterial RNA (2 µg) were directly labelled with Cy3 fluorophore using the Universal Linkage System (ULS, Kreatech Diagnostics). Microarray hybridisations were conducted as previously described using an M.tb complex pan-genome microarray generated by the Bacterial Microarray Group at St. George’s (ArrayExpress accession number A-BUGS-41).

INSTRUMENT(S): NA

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Simon Waddell 

PROVIDER: E-MTAB-3872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress