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Unknown,Transcriptomics,Genomics,Proteomics

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Physcomitrella patensTranscriptome or Gene expression

ABSTRACT: We used heat shock (HS) treatments and high-throughput mRNA sequencing to obtain HS transcriptomes in the moss Physcomitrella patens. Besides revealing differential gene expression at the transcriptional level, a transcriptome survey identified abundant AS events in the single-cellâtype, undifferentiated, moss protonemal cell. For preparing RNA-seq samples, protonemal colonies were grown at 38Â°C for 1 h (1st HS), recovered at 25Â°C for 5.5 h, and then grown at 38Â°C again for 1 h (2nd HS). Cells remaining at 25Â°C were collected as the control. Note: All samples in SRA were assigned the same sample accession (SRS402930). This is incorrect as there are different samples, hence âSource Nameâ was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Physcomitrella patens

SUBMITTER: Shih-Long Tu

PROVIDER: E-MTAB-4252 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-Wide Analysis of Heat-Sensitive Alternative Splicing in Physcomitrella patens.

Chang Chiung-Yun CY Lin Wen-Dar WD Tu Shih-Long SL

Plant physiology 20140428 2

Plant growth and development are constantly influenced by temperature fluctuations. To respond to temperature changes, different levels of gene regulation are modulated in the cell. Alternative splicing (AS) is a widespread mechanism increasing transcriptome complexity and proteome diversity. Although genome-wide studies have revealed complex AS patterns in plants, whether AS impacts the stress defense of plants is not known. We used heat shock (HS) treatments at nondamaging temperature and mess ...[more]

PMID: 24777346

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