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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Oryza sativa Transcriptome or Gene expression

ABSTRACT: The transcriptomes of resistant BPH15 introgression line and the susceptible recipient line were analyzed using high-throughput RNA sequencing. In total, 2,914 differentially expressed genes (DEGs) were identified. BPH-responsive transcript profiles were distinct between resistant and susceptible plants and between the early stage (6 h after infestation, HAI) and late stage (48 HAI). The key defense mechanism was related to jasmonate signaling, ethylene signaling, receptor kinase, MAPK cascades, Ca2+ signaling, PR genes, transcription factors, and protein post-translational modifications. Note: All samples in SRA were assigned the same sample accession (SRS565690 and SRS565691). This is incorrect as there are different samples, hence Source Name was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Oryza sativa

SUBMITTER: Wentang Lv

PROVIDER: E-MTAB-4293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.

Lv Wentang W Du Ba B Shangguan Xinxin X Zhao Yan Y Pan Yufang Y Zhu Lili L He Yuqing Y He Guangcun G

BMC genomics 20140811

<h4>Background</h4>Brown planthopper (BPH, Nilaparvata lugens Stål), is the most destructive phloem-feeding insect pest of rice (Oryza sativa). The BPH-resistance gene BPH15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. Nevertheless, molecular mechanism of the resistance remain unclear. In this study, we narrowed down the position of BPH15 on chromosome 4 and investigated the transcriptome of BPH15 rice after BPH attacked.<h4>Results</h4>We ...[more]

PMID: 25109872

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