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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of ESC derived hepatocytes treated with either vehicle or high energy substrates to model non alcoholic fatty liver disease

ABSTRACT: Female H9 ESCs were maintained on matrigel and differentiated into hepatocyte like cells as previously described (Szkolnicka D, Farnworth SL, Lucendo-Villarin B, Hay DC. Curr Protoc Stem Cell Biol. 2014 Aug 1;30:1G.5.1-12. doi: 10.1002/9780470151808.sc01g05s30) . Sodium L-Lactate (L7022, Sigma, Gillingham, UK) 10mM, Sodium Pyruvate 1mM (Sigma, Dorset, UK) and Octanoic Acid (C2875, Sigma, Gillingham, UK) 2mM for 48 hours before harvesting.

ORGANISM(S): Homo sapiens

SUBMITTER: Marcus Lyall

PROVIDER: E-MTAB-6227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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