Dataset Information

Isolation and Characterization of Carbapenem-Resistant Escherichia coli Carrying bla_NDM and mcr-1 from Recurrent Urinary Tract Infection Patient.

ABSTRACT:

Objective

The emergence of carbapenem-resistant E. coli (CRECO), leading to few antibacterial drugs available for CRECO infection. In this study, we report three carbapenem-resistant Escherichia coli (E. coli) isolates coproducing bla_NDM and mcr-1 from patients with recurrent urinary tract infection (RUTI). Carbapenem-resistant E. coli strains, E55, E84, and E85, were isolated from the urine sample of RUTI patients.

Methods

Antimicrobial susceptibility testing (AST) was conducted with VITEK-2 compact system and Kirby-Bauer (K-B) disk diffusion method. The ESBL test was detected by the disk diffusion method. The EDTA-modified carbapenem inactivation method (eCIM) and modified carbapenem inactivation method (mCIM) were performed for screening the carbapenemase. Multilocus sequence typing (MLST) was performed for molecular typing of the strains. The resistance genes were detected by PCR.

Results

The three isolates were all susceptible to tigecycline and nitrofurantoin. The bla_NDM-1, bla_CMY-6, bla_TEM-1 and bla_CTX-M-1, mcr-1, and porin loss expression of outer membrane protein F (OmpF) were detected in E55, which was assigned to ST2. The E84 and E85 were identified as ST471 carrying bla_NDM-5, bla_CTX-M55, and bla_TEM-1 and the quinsolone-resistant genes aac(6')-Ib-cr and mcr-1.

Conclusion

To our knowledge, our study is the first to report carbapenem-resistant E. coli strains carrying bla_NDM and mcr-1 from urine of the recurrent urinary tract infection patients. These E. coli strains carrying bla_NDM and mcr-1 should be closely monitored.

SUBMITTER: Chen L

PROVIDER: S-EPMC10482531 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Isolation and Characterization of Carbapenem-Resistant Escherichia coli Carrying blaNDM and mcr-1 from Recurrent Urinary Tract Infection Patient.

Chen Liang L Jian Jiyong J Xie Zeqiang Z Zhao Ping P Zhang Man M

The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale 20230830

<h4>Objective</h4>The emergence of carbapenem-resistant E. coli (CRECO), leading to few antibacterial drugs available for CRECO infection. In this study, we report three carbapenem-resistant Escherichia coli (E. coli) isolates coproducing blaNDM and mcr-1 from patients with recurrent urinary tract infection (RUTI). Carbapenem-resistant E. coli strains, E55, E84, and E85, were isolated from the urine sample of RUTI patients.<h4>Methods</h4>Anti ...[more]

PMID: 37680456

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