NF-κB c-Rel Is a Potential Therapeutic Target for Acute Corneal Transplant Rejection.

ABSTRACT:

Purpose

The purpose of this study was to determine the role of nuclear factor kappa B (NF-κB) c-Rel during acute corneal transplant rejection and whether targeting c-Rel can reduce corneal transplant rejection.

Methods

Allogeneic corneal transplantation was performed in wild-type and c-Rel-deficient mice. Corneal graft survival rate, opacity, neovascularization, and edema were evaluated by slit-lamp microscopy. Adeno-associated virus 6 (AAV6) expressing c-Rel-specific small hairpin RNA (AAV6-shRel) and the small-molecule compound pentoxifylline (PTXF) were used to reduce c-Rel expression. Enzyme-linked immunosorbent assay was used to determine the expression of inflammatory cytokines. c-Rel expression was determined by quantitative RT-PCR and western blot. The effect of c-Re

SUBMITTER: Zheng Q 

PROVIDER: S-EPMC10653260 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature