Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry.

ABSTRACT: The cell-derived vesicles (CDVs) obtained using a proprietary extrusion process are the foundation of BioDrone platform technology. With superior productivity and versatility, this technology has garnered increasing attention in broad applications, particularly as a drug delivery vehicle. Previously, we showed that CDVs exhibited varying levels of expression for tetraspanin and organelle membrane markers while revealing no discernible differences in physical characteristics compared to naturally produced extracellular vesicles (EVs). To further understand and utilize the therapeutic potentials of CDVs, a more comprehensive study of membrane protein profiles is necessary. In addition, it is crucial to validate that the CDVs produced from extrusion are indeed intact lipid vesicles rather tha

SUBMITTER: Lau HC 

PROVIDER: S-EPMC10762029 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature