Emergence of an IncX3 plasmid co-harbouring the carbapenemase genes bla_NDM-5 and bla_OXA-181.

ABSTRACT:

Background

The spread of transmissible plasmids with carbapenemase genes has contributed to a global increase in carbapenemase-producing Enterobacterales over the past two decades, with bla_NDM and bla_OXA among the most prevalent carbapenemase genes.

Objectives

To characterize an Escherichia coli isolate co-carrying bla_NDM-5 and bla_OXA-181 (JBEHAAB-19-0176) that was isolated in the Japan Antimicrobial Resistant Bacterial Surveillance in 2019-20, and to evaluate the functional advantage of carrying both genes as opposed to only one.

Methods

The whole-genome sequence of the isolate was determined using long- and short-read sequencing. Growth assay and co-culture experiments were performed for

SUBMITTER: Zuo H 

PROVIDER: S-EPMC11089413 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature