Dataset Information
Gut microbiota dysbiosis contributes to choline unavailability and NAFLD development.
Ontology highlight
ABSTRACT: Objectives
Non-alcoholic fatty Liver Disease (NAFLD) poses a growing global health concern, yet its complex aetiology remains incompletely understood. Emerging evidence implicates the gut microbiome and choline metabolism in NAFLD pathogenesis. This study aims to elucidate the association of choline-consuming bacteria in gut microbiome with choline level.Methods
A population comprising 85 NAFLD patients and 30 healthy controls was selected. DNA extraction from stool samples was conducted using the FavorPrep™ Stool DNA Isolation Mini Kit, followed by polymerase chain reaction (PCR) detection of choline-consuming bacterial strains and quantitative PCR (qPCR) for Cut C gene expression. Choline content measurement was performed using fluorescence high-performance liquid chromatography (FL-HPLC).Results
Our findings revealed a significant reduction in choline levels among NAFLD patients compared to healthy controls. ROC curve analysis demonstrated choline levels and Cut C expression as a promising diagnostic tool for NAFLD, with high sensitivity and specificity. The microbial analysis identified specific choline-consuming bacteria enriched in NAFLD patients, notably Anarococcus Hydrogenalis and Clostridium asparagiforme. This was consistent with higher Cut C gene expression in patients compared to healthy individuals, which is responsible for encoding an enzyme to consume choline by these bacteria.Conclusion
The current study gives a possible association between gut microbiota and the development of NAFLD, possibly due to an alteration in choline bioavailability. Further research is required to determine whether gut bacteria alter in the context of NAFLD or a change in their composition might lead to NAFLD progression, possibly via alternation in choline bioavailability.Supplementary information
The online version contains supplementary material available at 10.1007/s40200-024-01511-6.
SUBMITTER: Moradzad M
PROVIDER: S-EPMC11711859 | biostudies-literature | 2025 Jun
REPOSITORIES: biostudies-literature
Publications
Gut microbiota dysbiosis contributes to choline unavailability and NAFLD development.
Journal of diabetes and metabolic disorders 20250108 1
<h4>Objectives</h4>Non-alcoholic fatty Liver Disease (NAFLD) poses a growing global health concern, yet its complex aetiology remains incompletely understood. Emerging evidence implicates the gut microbiome and choline metabolism in NAFLD pathogenesis. This study aims to elucidate the association of choline-consuming bacteria in gut microbiome with choline level.<h4>Methods</h4>A population comprising 85 NAFLD patients and 30 healthy controls was selected. DNA extraction from stool samples was c ...[more]
