Form-deprivation myopia in chick induces limited changes in retinal gene expression.

ABSTRACT:

Purpose

Evidence has implicated the retina as a principal controller of refractive development. In the present study, the retinal transcriptome was analyzed to identify alterations in gene expression and potential signaling pathways involved in form-deprivation myopia of the chick.

Methods

One-week-old white Leghorn chicks wore a unilateral image-degrading goggle for 6 hours or 3 days (n = 6 at each time). Total RNA from the retina/(retinal pigment epithelium) was used for expression profiling with chicken gene microarrays (Chicken GeneChips; Affymetrix, Santa Clara, CA). To identify gene expression level differences between goggled and contralateral nongoggled eyes, normalized microarray signal intensities were analyzed by the significance analysis of microarrays (SAM) appr

SUBMITTER: McGlinn AM 

PROVIDER: S-EPMC1983368 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature