An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins.

ABSTRACT: The recombinant expression of integral membrane proteins is considered a major challenge, and together with the crystallization step, the major hurdle toward routine structure determination of membrane proteins. Basic methodologies for high-throughput (HTP) expression optimization of soluble proteins have recently emerged, providing statistically significant success rates for producing such proteins. Experimental procedures for handling integral membrane proteins are generally more challenging, and there have been no previous comprehensive reports of HTP technology for membrane protein production. Here, we present a generic and integrated parallel HTP strategy for cloning and expression screening of membrane proteins in their detergent solubilized form. Based on this strategy, we provide o

SUBMITTER: Eshaghi S 

PROVIDER: S-EPMC2279288 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature