Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma.

ABSTRACT: Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardize

SUBMITTER: Addona TA 

PROVIDER: S-EPMC2855883 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature