Protein interaction network topology uncovers melanogenesis regulatory network components within functional genomics datasets.

ABSTRACT:

Background

RNA-mediated interference (RNAi)-based functional genomics is a systems-level approach to identify novel genes that control biological phenotypes. Existing computational approaches can identify individual genes from RNAi datasets that regulate a given biological process. However, currently available methods cannot identify which RNAi screen "hits" are novel components of well-characterized biological pathways known to regulate the interrogated phenotype. In this study, we describe a method to identify genes from RNAi datasets that are novel components of known biological pathways. We experimentally validate our approach in the context of a recently completed RNAi screen to identify novel regulators of melanogenesis.

Results

In this study, we utilize a PPI network

SUBMITTER: Ho H 

PROVIDER: S-EPMC2904735 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature