Analysis of the sulfur-regulated control of the cystathionine γ-lyase gene of Neurospora crassa.

ABSTRACT:

Background

Cystathionine γ-lyase plays a key role in the transsulfuration pathway through its primary reaction of catalyzing the formation of cysteine from cystathionine. The Neurospora crassa cystathionine γ-lyase gene (cys-16(+)) is of particular interest in dissecting the regulation and dynamics of transsulfuration. The aim of this study was to determine the regulatory connection of cys-16(+) to the Neurospora sulfur regulatory network. In addition, the cys-16(+) promoter was characterized with the goal of developing a strongly expressed and regulatable gene expression tool.

Findings

The cystathionine γ-lyase cys-16(+) gene was cloned and characterized. The gene, which contains no introns, encodes a protein of 417 amino acids with conserved pyridoxal 5'-phosphate binding

SUBMITTER: Reveal BS 

PROVIDER: S-EPMC3496659 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature