Dataset Information

Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.

ABSTRACT:

Objective

To determine whether peripheral blood stem cell transplant (PBSCT) result in engraftment of donor stem cells in the recipient uterus.

Design

Prospective clinical and laboratory research.

Setting

Translational medicine research hospital. PATIENT(S)/ANIMAL(S): Macaque and human bone marrow transplant recipients.

Intervention(s)

Rhesus macaques received autologous transduced immunoselected cytokine-mobilized CD34+ cells after total body irradiation. Vector constructs expressed green fluorescent protein. In the human subjects, prior PBSCT subjects underwent endometrial biopsy and bone marrow aspiration. Macaque and human endometrial and bone marrow cells were isolated and cultured. Fluorescent microscopy, flow cytometry, and polymerase chain reaction (PCR) were used to evaluate for the presence of donor-derived cells.

Main outcome measure(s)

Presence of donor cells in recipient endometrium and bone marrow stroma.

Result(s)

The macaque endometrial cells did not exhibit evidence of green fluorescent protein labeling. Human endometrial cells were cultured and the absence of donor blood contamination was verified. The PCR evaluation of the human endometrial cells did not demonstrate evidence of donor short tandem repeats.

Conclusion(s)

The PBSCT did not result in engraftment of donor-derived cells in the endometrium.

SUBMITTER: Wolff EF

PROVIDER: S-EPMC3563919 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.

Wolff Erin F EF Uchida Naoya N Donahue Robert E RE Metzger Mark E ME Hsieh Matthew M MM Libfraind Lauren L LL Hill Micah J MJ Tisdale John F JF

Fertility and sterility 20121025 2

<h4>Objective</h4>To determine whether peripheral blood stem cell transplant (PBSCT) result in engraftment of donor stem cells in the recipient uterus.<h4>Design</h4>Prospective clinical and laboratory research.<h4>Setting</h4>Translational medicine research hospital. PATIENT(S)/ANIMAL(S): Macaque and human bone marrow transplant recipients.<h4>Intervention(s)</h4>Rhesus macaques received autologous transduced immunoselected cytokine-mobilized CD34+ cells after total body irradiation. Vector con ...[more]

PMID: 23103021

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Project description:Study questionCan endometrial stromal stem/progenitor cell markers, SUSD2 and CD146/CD140b, enrich for human myometrial and fibroid stem/progenitor cells?Summary answerSUSD2 enriches for myometrial and fibroid cells that have mesenchymal stem cell (MSC) characteristics and can also be induced to decidualise.What is known alreadyMesenchymal stem-like cells have been separately characterised in the endometrial stroma and myometrium and may contribute to diseases in their respective tissues.Study design, size, durationNormal myometrium, fibroids and endometrium were collected from hysterectomies with informed consent. Primary cells or tissues were used from at least three patient samples for each experiment.Participants/materials, setting, methodsFlow cytometry, immunohistochemistry and immunofluorescence were used to characterise tissues. In vitro colony formation in normoxic and hypoxic conditions, MSC lineage differentiation (osteogenic and adipogenic) and decidualisation were used to assess stem cell activity. Xenotransplantation into immunocompromised mice was used to determine in vivo stem-like activity. Endpoint measures included quantitative PCR, colony formation, trichrome, Oil Red O and alkaline phosphatase activity staining.Main results and the role of chanceCD146+CD140b+ and/or SUSD2+ myometrial and fibroid cells were located in the perivascular region and formed more colonies in vitro compared to control cells and differentiated down adipogenic and osteogenic mesenchymal lineages in vitro. SUSD2+ myometrial cells had greater in vitro decidualisation potential, and SUSD2+ fibroid cells formed larger tumours in vivo compared to control cells.Large-scale dataN/A.Limitations, reasons for cautionMarkers used in this study enrich for cells with stem/progenitor cell activity; however, they do not distinguish stem from progenitor cells. SUSD2+ myometrial cells express markers of decidualisation when treated in vitro, but in vivo assays are needed to fully demonstration their ability to decidualise.Wider implications of the findingsThese results suggest a possible common MSC for the endometrial stroma and myometrium, which could be the tumour-initiating cell for uterine fibroids.Study funding/competing interest(s)These studies were supported by NIH grants to JMT (R01OD012206) and to ALP (F32HD081856). The authors certify that we have no conflicts of interest to disclose.

Dataset Information

Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.

Objective

Design

Setting

Intervention(s)

Main outcome measure(s)

Result(s)

Conclusion(s)

Publications

Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.

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