Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.

ABSTRACT:

Objective

To determine whether peripheral blood stem cell transplant (PBSCT) result in engraftment of donor stem cells in the recipient uterus.

Design

Prospective clinical and laboratory research.

Setting

Translational medicine research hospital. PATIENT(S)/ANIMAL(S): Macaque and human bone marrow transplant recipients.

Intervention(s)

Rhesus macaques received autologous transduced immunoselected cytokine-mobilized CD34+ cells after total body irradiation. Vector constructs expressed green fluorescent protein. In the human subjects, prior PBSCT subjects underwent endometrial biopsy and bone marrow aspiration. Macaque and human endometrial and bone marrow cells were isolated and cultured. Fluorescent microscopy, flow cytometry, and polymerase chain reaction (PCR

SUBMITTER: Wolff EF 

PROVIDER: S-EPMC3563919 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature