Dataset Information

Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration.

ABSTRACT:

Background

DBS are an increasingly common clinical matrix.

Methods & results

Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood.

Conclusion

DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.

SUBMITTER: Ellefsen KN

PROVIDER: S-EPMC4644129 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration.

Ellefsen Kayla N KN da Costa Jose Luiz JL Concheiro Marta M Anizan Sebastien S Barnes Allan J AJ Pirard Sandrine S Gorelick David A DA Huestis Marilyn A MA

Bioanalysis 20150101 16

<h4>Background</h4>DBS are an increasingly common clinical matrix.<h4>Methods & results</h4>Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoyle ...[more]

PMID: 26327184

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Project description:RationaleInitial sensitivity to drugs of abuse often predicts subsequent use and abuse, but this relationship is not always observed in human studies. Moreover, studies examining the relationship between initial locomotor sensitivity and the rewarding and reinforcing effects of drugs in animal models have also been equivocal. Understanding the relationship between initial drug effects and propensity to continue use, potentially resulting in the development of a substance use disorder, may help to identify key targets for prevention and treatment.ObjectivesWe examined intravenous cocaine self-administration in a set of mouse strains that were previously identified to be at the phenotypic extremes for cocaine-induced locomotor activation to determine if initial locomotor sensitivity predicted acquisition, extinction, dose response, or progressive ratio (PR) breakpoint.MethodsWe selected eight inbred mouse strains based on locomotor sensitivity to 20 mg/kg cocaine. These strains, designated as low and high responders, were tested in an intravenous self-administration paradigm that included acquisition of 0.5 mg/(kg*inf) under a FR1 schedule, extinction, re-acquisition, dose response to 0.125, 0.25, 0.5, 1, and 2 mg/(kg*inf), and progressive ratio.ResultsWe observed overall differences in self-administration behavior between high and low responders. Low responders self-administered less cocaine and had lower breakpoints under the PR schedule. However, we also observed strain differences within each group. Self-administration in the low responder, LG/J, more closely resembled the behavior of the high-responding group, and the high responder, P/J, had self-administration behavior that more closely resembled the low-responding group.ConclusionsWe conclude that acute cocaine-induced locomotor activation does predict self-administration behavior, but in a strain-specific manner. These data support the idea that genetic background influences the relationship among addiction-related behaviors.

Dataset Information

Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration.

Background

Methods & results

Conclusion

Publications

Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration.

Similar Datasets

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